› 4ub9

X-ray diffraction
2.27Å resolution

Structural and catalytic characterization of molinate hydrolase

Released:
DOI: 10.2210/pdb4ub9/pdb
PDB entry 4ub9 coloured by chain, front view.
PDB entry 4ub9 coloured by chain, side view.
PDB entry 4ub9 coloured by chain, top view.
Source organism: Gulosibacter molinativorax
Primary publication:
Structure-guided engineering of molinate hydrolase for the degradation of thiocarbamate pesticides.
Leite JP, Duarte M, Paiva AM, Ferreira-da-Silva F, Matias PM, Nunes OC, Gales L
PLoS One 10 e0123430 (2015)
PMID: 25905461

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidohydrolase-related domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 496 amino acids
Theoretical weight: 54.68 KDa
Source organism: Gulosibacter molinativorax
Expression system: Escherichia coli
UniProt:
  • Canonical: G2XLB0 (Residues: 2-465; Coverage: 100%)
Gene name: molA
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1, ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 367.696Å b: 99.079Å c: 131.337Å
α: 90° β: 109.61° γ: 90°
R-values:
R R work R free
0.202 0.201 0.229
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Production of microparticles of molinate degrading biocatalysts using the spray drying technique.
Lopes et al. (2016)
1 more

1 mention without citation

Structure-guided engineering of molinate hydrolase for the degradation of thiocarbamate pesticides.
Leite et al. (2015)