4ub0

X-ray diffraction
2.2Å resolution

New design for monovalent bispecific IgG through cysteine engineering of the CH1-CL interface

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
IgG1, heavy chain Chain: H
Molecule details ›
Chain: H
Length: 220 amino acids
Theoretical weight: 23.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
IgG1, light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.47 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2
Unit cell:
a: 126.154Å b: 64.045Å c: 82.395Å
α: 90° β: 129.09° γ: 90°
R-values:
R R work R free
0.233 0.232 0.252
Expression system: Homo sapiens