› 4uat

X-ray diffraction
1.3Å resolution

Crystal structure of CbbY (mutant D10N) from Rhodobacter sphaeroides in complex with Xylulose-(1,5)bisphosphate, crystal form I

Released:
DOI: 10.2210/pdb4uat/pdb
PDB 4uat coloured by chain and viewed from the front.
PDB 4uat coloured by chain and viewed from the side.
PDB 4uat coloured by chain and viewed from the top.
Source organism: Cereibacter sphaeroides
Primary publication:
Degradation of potent Rubisco inhibitor by selective sugar phosphatase.
Bracher A, Sharma A, Starling-Windhof A, Hartl FU, Hayer-Hartl M
Nat Plants 1 14002 (2015)
PMID: 27246049

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein CbbY Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 25.15 KDa
Source organism: Cereibacter sphaeroides
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P95649 (Residues: 1-230; Coverage: 100%)
Gene name: cbbY
Sequence domains: Haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand XBP 2 x XBP
Ligand MG 2 x MG
Ligand MES 2 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 35.419Å b: 51.468Å c: 122.083Å
α: 90° β: 94.06° γ: 90°
R-values:
R R work R free
0.124 0.121 0.168
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Increasing metabolic potential: C-fixation.
Andralojc et al. (2018)
4 more

1 mention without citation

From chaperonins to Rubisco assembly and metabolic repair.
Hayer-Hartl M. (2017)