› 4uao

X-ray diffraction
3.1Å resolution

Crystal structure of Apical Membrane Antigen 1 from Plasmodium Knowlesi in complex with an invasion inhibitory antibody

Released:
DOI: 10.2210/pdb4uao/pdb
PDB entry 4uao coloured by chain, front view.
PDB entry 4uao coloured by chain, side view.
PDB entry 4uao coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of Plasmodium knowlesi apical membrane antigen 1 and its complex with an invasion-inhibitory monoclonal antibody.
Vulliez-Le Normand B, Faber BW, Saul FA, van der Eijk M, Thomas AW, Singh B, Kocken CH, Bentley GA
PLoS One 10 e0123567 (2015)
PMID: 25886591

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-104749 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Apical membrane antigen 1 Chain: A
Molecule details ›
Chain: A
Length: 370 amino acids
Theoretical weight: 42.51 KDa
Source organism: Plasmodium knowlesi
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A384KGX8 (Residues: 43-387; Coverage: 61%)
Gene name: PKNH_0931500
Sequence domains: Apical membrane antigen 1
Structure domains: Hepatocyte Growth Factor
Ig kappa chain C region, B allele Chain: B
Molecule details ›
Chain: B
Length: 214 amino acids
Theoretical weight: 23.67 KDa
Source organism: Rattus norvegicus
UniProt:
  • Canonical: P01835 (Residues: 1-106; Coverage: 100%)
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Ig gamma-2A chain C region Chain: C
Molecule details ›
Chain: C
Length: 223 amino acids
Theoretical weight: 23.97 KDa
Source organism: Rattus norvegicus
UniProt:
  • Canonical: P20760 (Residues: 1-102; Coverage: 32%)
Gene name: Igg-2a
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 165.864Å b: 71.807Å c: 90.688Å
α: 90° β: 116.37° γ: 90°
R-values:
R R work R free
0.192 0.188 0.266
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

Plasmodium knowlesi malaria: current research perspectives.
Amir et al. (2018)
1 more

3 mentions without citation

Crystal structure of Plasmodium knowlesi apical membrane antigen 1 and its complex with an invasion-inhibitory monoclonal antibody.
Vulliez-Le Normand et al. (2015)
2 more