› 4uaj

X-ray diffraction
2.7Å resolution

Crystal structure of NqrF in hexagonal space group

Released:
DOI: 10.2210/pdb4uaj/pdb
PDB 4uaj coloured by chain and viewed from the front.
PDB 4uaj coloured by chain and viewed from the side.
PDB 4uaj coloured by chain and viewed from the top.
Source organism: Vibrio cholerae
Primary publication:
Structure of the V. cholerae Na+-pumping NADH:quinone oxidoreductase.
Steuber J, Vohl G, Casutt MS, Vorburger T, Diederichs K, Fritz G
Nature 516 62-7 (2014)
PMID: 25471880

Function and Biology Details

Reaction catalysed: 
NADH + ubiquinone + n Na(+)(Side 1) = NAD(+) + ubiquinol + n Na(+)(Side 2)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+)-translocating NADH-quinone reductase subunit F Chain: A
Molecule details ›
Chain: A
Length: 284 amino acids
Theoretical weight: 32.33 KDa
Source organism: Vibrio cholerae
Expression system: Vibrio cholerae
UniProt:
  • Canonical: A5F5Y4 (Residues: 129-408; Coverage: 69%)
Gene names: VC0395_A1879, VC395_2406, nqrF
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FAD 1 x FAD
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P6522
Unit cell:
a: 145.22Å b: 145.22Å c: 90.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.189 0.233
Expression system: Vibrio cholerae

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Citations

6 review citations

Architecture of bacterial respiratory chains.
Kaila et al. (2021)
5 more