4u9g

X-ray diffraction
2.25Å resolution

Crystal structure of an H-NOX protein from S. oneidensis in the Fe(II)CO ligation state, Q154A/Q155A/K156A mutant

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HNOB domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 187 amino acids
Theoretical weight: 21.16 KDa
Source organism: Shewanella oneidensis MR-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8EF49 (Residues: 1-181; Coverage: 100%)
Gene name: SO_2144
Sequence domains: Haem-NO-binding
Structure domains: H-NOX domain

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P6322
Unit cell:
a: 164.173Å b: 164.173Å c: 102.366Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.182 0.193
Expression system: Escherichia coli