› 4u9d

X-ray diffraction
2.5Å resolution

Crystal Structure of the Zn-directed tetramer of the engineered cyt cb562 variant, AB3

Released:
DOI: 10.2210/pdb4u9d/pdb
PDB 4u9d coloured by chain and viewed from the front.
PDB 4u9d coloured by chain and viewed from the side.
PDB 4u9d coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
A designed supramolecular protein assembly with in vivo enzymatic activity.
Song WJ, Tezcan FA
Science 346 1525-8 (2014)
PMID: 25525249

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 106 amino acids
Theoretical weight: 11.84 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562
Structure domains: Cytochrome c/b562

Ligands and Environments

2 bound ligands:
Ligand HEC 4 x HEC
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P61
Unit cell:
a: 54.83Å b: 54.83Å c: 237.82Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.238 0.296
Expression system: Escherichia coli BL21(DE3)

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Citations

27 review citations

Supramolecular assembly of protein building blocks: from folding to function.
Kim et al. (2022)
26 more