› 4u98

X-ray diffraction
1.15Å resolution

Structure of mycobacterial maltokinase, the missing link in the essential GlgE-pathway (AppCp complex)

Released:
DOI: 10.2210/pdb4u98/pdb
PDB 4u98 coloured by chain and viewed from the front.
PDB 4u98 coloured by chain and viewed from the side.
PDB 4u98 coloured by chain and viewed from the top.
Source organism: Mycolicibacterium vanbaalenii PYR-1
Primary publication:
Structure of mycobacterial maltokinase, the missing link in the essential GlgE-pathway.
Fraga J, Maranha A, Mendes V, Pereira PJ, Empadinhas N, Macedo-Ribeiro S
Sci Rep 5 8026 (2015)
PMID: 25619172

Function and Biology Details

Reaction catalysed: 
ATP + maltose = ADP + alpha-maltose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Maltokinase Chain: A
Molecule details ›
Chain: A
Length: 454 amino acids
Theoretical weight: 49.75 KDa
Source organism: Mycolicibacterium vanbaalenii PYR-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A1TH50 (Residues: 1-441; Coverage: 100%)
Gene names: Mvan_5735, mak
Sequence domains:
Structure domains: Aminoglycoside 3'-phosphotransferase; Chain: A, domain 2

Ligands and Environments

2 bound ligands:
Ligand ACP 1 x ACP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P2221
Unit cell:
a: 63Å b: 73.52Å c: 106.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.145 0.163
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Using on-board sound recordings to infer behaviour of free-moving wild animals.
Greif et al. (2019)
2 more

2 mentions without citation

Structure of mycobacterial maltokinase, the missing link in the essential GlgE-pathway.
Fraga et al. (2015)
1 more