› 4u8g

X-ray diffraction
2.9Å resolution

Crystal structure of 2-keto-3-deoxy-D-gluconate dehydrogenase from Streptococcus agalactiae

Released:
DOI: 10.2210/pdb4u8g/pdb
PDB entry 4u8g coloured by chain, front view.
PDB entry 4u8g coloured by chain, side view.
PDB entry 4u8g coloured by chain, top view.
Source organism: Streptococcus agalactiae NEM316
Primary publication:
Metabolic fate of unsaturated glucuronic/iduronic acids from glycosaminoglycans: molecular identification and structure determination of streptococcal isomerase and dehydrogenase.
Maruyama Y, Oiki S, Takase R, Mikami B, Murata K, Hashimoto W
J Biol Chem 290 6281-92 (2015)
PMID: 25605731

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184294 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gluconate 5-dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 278 amino acids
Theoretical weight: 29.94 KDa
Source organism: Streptococcus agalactiae NEM316
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8E370 (Residues: 1-270; Coverage: 100%)
Gene name: gbs1891
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P6222
Unit cell:
a: 83.943Å b: 83.943Å c: 181.101Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.211 0.287
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Glycan-metabolizing enzymes in microbe-host interactions: the Streptococcus pneumoniae paradigm.
Hobbs et al. (2018)
1 more

1 mention without citation

Metabolic fate of unsaturated glucuronic/iduronic acids from glycosaminoglycans: molecular identification and structure determination of streptococcal isomerase and dehydrogenase.
Maruyama et al. (2015)