› 4u8f

X-ray diffraction
1.55Å resolution

Crystal structure of 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase complexed with a tartrate

Released:
DOI: 10.2210/pdb4u8f/pdb
PDB entry 4u8f coloured by chain, front view.
PDB entry 4u8f coloured by chain, side view.
PDB entry 4u8f coloured by chain, top view.
Source organism: Streptococcus agalactiae NEM316
Primary publication:
Metabolic fate of unsaturated glucuronic/iduronic acids from glycosaminoglycans: molecular identification and structure determination of streptococcal isomerase and dehydrogenase.
Maruyama Y, Oiki S, Takase R, Mikami B, Murata K, Hashimoto W
J Biol Chem 290 6281-92 (2015)
PMID: 25605731

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184293 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribose-5-phosphate isomerase C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 220 amino acids
Theoretical weight: 24.57 KDa
Source organism: Streptococcus agalactiae NEM316
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8E369 (Residues: 1-212; Coverage: 100%)
Gene name: gbs1892
Sequence domains:
Structure domains: Sugar-phosphate isomerase, RpiB/LacA/LacB

Ligands and Environments

1 bound ligand:
Ligand TLA 2 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P41212
Unit cell:
a: 92.955Å b: 92.955Å c: 129.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.211
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Glycan-metabolizing enzymes in microbe-host interactions: the Streptococcus pneumoniae paradigm.
Hobbs et al. (2018)
1 more

1 mention without citation

Metabolic fate of unsaturated glucuronic/iduronic acids from glycosaminoglycans: molecular identification and structure determination of streptococcal isomerase and dehydrogenase.
Maruyama et al. (2015)