4u7j

X-ray diffraction
1.75Å resolution

Crystal structure of Argininosuccinate synthase from Mycobacterium thermoresistibile

Released:
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed:
ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Argininosuccinate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 408 amino acids
Theoretical weight: 45.18 KDa
Source organism: Mycolicibacterium thermoresistibile ATCC 19527
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G7CBN9 (Residues: 1-400; Coverage: 100%)
Gene names: KEK_01915, argG
Sequence domains: Arginosuccinate synthase
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21212
Unit cell:
a: 93.94Å b: 144.44Å c: 57.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.146 0.182
Expression system: Escherichia coli BL21(DE3)