4u6p

X-ray diffraction
2.59Å resolution

Structural mechanism of error-free bypass of major benzo[a]pyrene adduct by human polymerase kappa

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase kappa Chains: A, B
Molecule details ›
Chains: A, B
Length: 526 amino acids
Theoretical weight: 60.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBT6 (Residues: 1-526; Coverage: 61%)
Gene names: DINB1, POLK
Sequence domains:
Structure domains:
DNA (5'-D(P*GP*CP*GP*GP*AP*TP*CP*AP*C)-3') Chains: C, P
Molecule details ›
Chains: C, P
Length: 9 nucleotides
Theoretical weight: 2.74 KDa
Source organism: unidentified
Expression system: Not provided
DNA (5'-D(*TP*AP*TP*GP*GP*TP*GP*AP*TP*CP*CP*GP*C)-3') Chains: D, T
Molecule details ›
Chains: D, T
Length: 13 nucleotides
Theoretical weight: 3.98 KDa
Source organism: unidentified
Expression system: Not provided

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 66.15Å b: 129.286Å c: 167.713Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.2 0.239
Expression systems:
  • Escherichia coli
  • Not provided