X-ray diffraction
2.1Å resolution

Structure of the periplasmic output domain of the Legionella pneumophila LapD ortholog CdgS9 in complex with Pseudomonas fluorescens LapG


Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Two component histidine kinase, GGDEF domain protein/EAL domain protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 131 amino acids
Theoretical weight: 14.84 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli
  • Canonical: Q5ZXA3 (Residues: 22-152; Coverage: 21%)
Gene name: lpg0829
Sequence domains: LapD/MoxY periplasmic domain
Putative cystine protease Chains: E, F
Molecule details ›
Chains: E, F
Length: 197 amino acids
Theoretical weight: 22.5 KDa
Source organism: Pseudomonas fluorescens Pf0-1
Expression system: Escherichia coli
  • Canonical: Q3KK32 (Residues: 51-247; Coverage: 79%)
Gene names: Pfl01_0130, lapF
Sequence domains: Bacterial transglutaminase-like cysteine proteinase BTLCP
Structure domains: C8orf32 fold

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21
Unit cell:
a: 75.854Å b: 73.674Å c: 88.223Å
α: 90° β: 92.88° γ: 90°
R R work R free
0.171 0.17 0.211
Expression system: Escherichia coli