4u4p

X-ray diffraction
1.89Å resolution

Crystal structure of the human condensin SMC hinge domain heterodimer with short coiled coils

Released:
Source organism: Homo sapiens
Entry authors: Uchiyama S, Kawahara K, Hosokawa Y, Fukakusa S, Oki H, Nakamura S, Noda M, Takino R, Miyahara Y, Maruno T, Kobayashi Y, Ohkubo T, Fukui K

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Structural maintenance of chromosomes protein 2 Chain: A
Molecule details ›
Chain: A
Length: 233 amino acids
Theoretical weight: 26.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95347 (Residues: 476-707; Coverage: 19%)
Gene names: CAPE, PRO0324, SMC2, SMC2L1
Sequence domains: SMC proteins Flexible Hinge Domain
Structural maintenance of chromosomes protein 4 Chain: B
Molecule details ›
Chain: B
Length: 247 amino acids
Theoretical weight: 27.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NTJ3 (Residues: 566-809; Coverage: 19%)
Gene names: CAPC, SMC4, SMC4L1
Sequence domains: SMC proteins Flexible Hinge Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 50.4Å b: 93.98Å c: 102.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.235
Expression system: Escherichia coli