4u2f

X-ray diffraction
1.8Å resolution

Crystal structure of dienelactone hydrolase B-1 variant (Q35H, F38L, Y64H, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G and G211D) at 1.80 A resolution

Released:

Function and Biology Details

Reaction catalysed:
4-carboxymethylenebut-2-en-4-olide + H(2)O = 4-oxohex-2-enedioate
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxymethylenebutenolidase Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 25.18 KDa
Source organism: Pseudomonas knackmussii
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: P0A115 (Residues: 1-236; Coverage: 100%)
Gene name: clcD
Sequence domains: Dienelactone hydrolase family
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P212121
Unit cell:
a: 48.934Å b: 70.295Å c: 74.264Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.218
Expression system: Escherichia coli DH5[alpha]