› 4u28

X-ray diffraction
1.33Å resolution

Crystal structure of apo Phosphoribosyl isomerase A from Streptomyces sviceus ATCC 29083

Released:
DOI: 10.2210/pdb4u28/pdb
PDB entry 4u28 coloured by chain, front view.
PDB entry 4u28 coloured by chain, side view.
PDB entry 4u28 coloured by chain, top view.
Source organism: Streptomyces sviceus ATCC 29083
Primary publication:
Co-occurrence of analogous enzymes determines evolution of a novel (βα)8-isomerase sub-family after non-conserved mutations in flexible loop.
Verduzco-Castro EA, Michalska K, Endres M, Juárez-Vazquez AL, Noda-García L, Chang C, Henry CS, Babnigg G, Joachimiak A, Barona-Gómez F
Biochem J 473 1141-52 (2016)
PMID: 26929404

Function and Biology Details

Reactions catalysed: 
1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110214 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosyl isomerase A Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 25.94 KDa
Source organism: Streptomyces sviceus ATCC 29083
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B5I4P8 (Residues: 1-245; Coverage: 100%)
Gene names: SSEG_10012, hisA, priA
Sequence domains: Histidine biosynthesis protein
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6522
Unit cell:
a: 69.111Å b: 69.111Å c: 175.036Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.128 0.127 0.151
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

How enzyme promiscuity and horizontal gene transfer contribute to metabolic innovation.
Glasner et al. (2020)
3 other articles

2 mentions without citation

The Role of Gene Elongation in the Evolution of Histidine Biosynthetic Genes.
Del Duca et al. (2020)
1 more