4u1t

X-ray diffraction
2Å resolution

The crystal structure of holo CalE6, a methionine gamma lyase from Micromonospora echinospora

Released:

Function and Biology Details

Reaction catalysed:
(1a) L-methionine = methanethiol + 2-aminobut-2-enoate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CalE6 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 389 amino acids
Theoretical weight: 41.98 KDa
Source organism: Micromonospora echinospora
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8KNG3 (Residues: 1-381; Coverage: 100%)
Gene name: calE6
Sequence domains: Cys/Met metabolism PLP-dependent enzyme
Structure domains:

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: I222
Unit cell:
a: 146.17Å b: 146.22Å c: 348.091Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.152 0.184
Expression system: Escherichia coli