› 4u1g

X-ray diffraction
3.1Å resolution

Plasmodium falciparum reticulocyte-binding protein homologue 5 (PfRH5) bound to monoclonal antibody QA1

Released:
DOI: 10.2210/pdb4u1g/pdb
PDB entry 4u1g coloured by chain, front view.
PDB entry 4u1g coloured by chain, side view.
PDB entry 4u1g coloured by chain, top view.
Source organisms:
Primary publication:
Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies.
Wright KE, Hjerrild KA, Bartlett J, Douglas AD, Jin J, Brown RE, Illingworth JJ, Ashfield R, Clemmensen SB, de Jongh WA, Draper SJ, Higgins MK
Nature 515 427-30 (2014)
PMID: 25132548

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207642 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Rh5 coiled-coil domain-containing protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 526 amino acids
Theoretical weight: 63.13 KDa
Source organism: Plasmodium falciparum
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: B2L3N7 (Residues: 1-526; Coverage: 100%)
Sequence domains: Rh5 coiled-coil domain
QA1 monoclonal antibody heavy chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 258 amino acids
Theoretical weight: 27.64 KDa
Source organism: Mus musculus
Expression system: Oryctolagus cuniculus
Structure domains: Immunoglobulins
QA1 monoclonal antibody light chain Chains: C, F
Molecule details ›
Chains: C, F
Length: 238 amino acids
Theoretical weight: 26.44 KDa
Source organism: Mus musculus
Expression system: Oryctolagus cuniculus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Unit cell:
a: 65.12Å b: 137.3Å c: 228.579Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.236 0.281
Expression systems:
  • Drosophila melanogaster
  • Oryctolagus cuniculus

Quick links

Citations

42 review citations

Merozoite surface proteins in red blood cell invasion, immunity and vaccines against malaria.
Beeson et al. (2016)
41 more

7 mentions without citation

The apicomplexan glideosome and adhesins - Structures and function.
Boucher et al. (2015)
6 more