4u14

X-ray diffraction
3.57Å resolution

Structure of the M3 muscarinic acetylcholine receptor bound to the antagonist tiotropium crystallized with disulfide-stabilized T4 lysozyme (dsT4L)

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; Muscarinic acetylcholine receptor M3 Chain: A
Molecule details ›
Chain: A
Length: 460 amino acids
Theoretical weight: 52.68 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08483 (Residues: 57-259, 482-563; Coverage: 48%)
  • Canonical: P00720 (Residues: 1-161; Coverage: 98%)
Gene names: Chrm-3, Chrm3, E
Sequence domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P41212
Unit cell:
a: 54.952Å b: 54.952Å c: 348.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.275 0.272 0.325
Expression system: Spodoptera frugiperda