› 4u0z

X-ray diffraction
2.95Å resolution

Eukaryotic Fic Domain containing protein with bound APCPP

Released:
DOI: 10.2210/pdb4u0z/pdb
PDB 4u0z coloured by chain and viewed from the front.
PDB 4u0z coloured by chain and viewed from the side.
PDB 4u0z coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Crystal Structure of the Human, FIC-Domain Containing Protein HYPE and Implications for Its Functions.
Bunney TD, Cole AR, Broncel M, Esposito D, Tate EW, Katan M
Structure (2014)
PMID: 25435325

Function and Biology Details

Reaction catalysed: 
ATP + [protein]-L-threonine = diphosphate + [protein]-O(4)-(5'-adenylyl)-L-threonine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein adenylyltransferase FICD Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 344 amino acids
Theoretical weight: 39.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BVA6 (Residues: 102-445; Coverage: 75%)
Gene names: FICD, HIP13, HYPE, UNQ3041/PRO9857
Sequence domains: Fic/DOC family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand APC 8 x APC
Ligand MG 8 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 77.1Å b: 83.754Å c: 130.023Å
α: 89.92° β: 89.57° γ: 89.43°
R-values:
R R work R free
0.208 0.206 0.251
Expression system: Escherichia coli

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Citations

5 review citations

Early Events in the Endoplasmic Reticulum Unfolded Protein Response.
Preissler et al. (2019)
4 more

1 mention without citation

Crystallographically correct but confusing presentation of structural models deposited in the Protein Data Bank.
Dauter et al. (2018)