4tz9

X-ray diffraction
2.13Å resolution

Structure of Metallo-beta-lactamase

Released:
Source organism: Escherichia coli
Entry authors: Ferguson JA, Brem J, Makena A, McDonough AM, Schofield CJ

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactamase_B domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 231 amino acids
Theoretical weight: 24.34 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I3VKD5 (Residues: 42-270; Coverage: 85%)
Gene names: NDM-3, blaNDM-3
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: I422
Unit cell:
a: 120.908Å b: 120.908Å c: 90.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.229 0.248
Expression system: Escherichia coli BL21(DE3)