X-ray diffraction
2.2Å resolution

DEAD-box helicase Mss116 bound to ssRNA and ADP-BeF


Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
ATP-dependent RNA helicase MSS116, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 509 amino acids
Theoretical weight: 57.79 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
  • Canonical: P15424 (Residues: 88-595; Coverage: 77%)
Gene names: MSS116, YD9346.05C, YDR194C
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases
RNA (5'-R(P*AP*AP*AP*AP*AP*AP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 7 nucleotides
Theoretical weight: 2.26 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21212
Unit cell:
a: 89.829Å b: 126.26Å c: 55.545Å
α: 90° β: 90° γ: 90°
R R work R free
0.218 0.216 0.254
Expression systems:
  • Escherichia coli
  • Not provided