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Ensemble refinement of the E502A variant of sacteLam55A from Streptomyces sp. SirexAA-E in complex with glucose

X-ray diffraction
1.75Å resolution
PDB entry 4tyv coloured by chain, ensemble of 25 models, front view.
PDB entry 4tyv coloured by chain, ensemble of 25 models, side view.
PDB entry 4tyv coloured by chain, ensemble of 25 models, top view.
Source organism: Streptomyces sp. SirexAA-E
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1
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Assembly Name: Exo-beta-1,3-glucanase
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Multimeric state: monomeric
Accessible surface area: 17622.86 Å2
Buried surface area: 441.38 Å2
Dissociation area: 111.54 Å2
Dissociation energy (ΔGdiss): -5.99 kcal/mol
Dissociation entropy (TΔSdiss): 0.05 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-124265
Assembly 2 (preferred)
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Assembly Name: Exo-beta-1,3-glucanase
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Multimeric state: monomeric
Accessible surface area: 18255.34 Å2
Buried surface area: 1022.3 Å2
Dissociation area: 107.77 Å2
Dissociation energy (ΔGdiss): -6.06 kcal/mol
Dissociation entropy (TΔSdiss): 0.04 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-124265

Macromolecules

Exo-beta-1,3-glucanase
Chains: A, B
Length: 551 amino acids
Theoretical weight: 59.08 KDa
Source organism: Streptomyces sp. SirexAA-E
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G2NFJ9 (Residues: 55-605; Coverage: 98%)
Gene name: SACTE_4363
InterPro: Pectin lyase fold
Exo-beta-1,3-glucanase in PDB entry 4tyv, assembly 2, front view.
Exo-beta-1,3-glucanase in PDB entry 4tyv, assembly 2, side view.
Exo-beta-1,3-glucanase in PDB entry 4tyv, assembly 2, top view.
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