X-ray diffraction
1.75Å resolution

Ensemble refinement of the E502A variant of sacteLam55A from Streptomyces sp. SirexAA-E in complex with glucose


Function and Biology Details

Reaction catalysed:
Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Exo-beta-1,3-glucanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 551 amino acids
Theoretical weight: 59.08 KDa
Source organism: Streptomyces sp. SirexAA-E
Expression system: Escherichia coli BL21(DE3)
  • Canonical: G2NFJ9 (Residues: 55-605; Coverage: 98%)
Gene name: SACTE_4363

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 53.939Å b: 100.055Å c: 99.556Å
α: 90° β: 92.31° γ: 90°
R R work R free
0.112 0.11 0.143
Expression system: Escherichia coli BL21(DE3)