4twr

X-ray diffraction
1.9Å resolution

Structure of UDP-glucose 4-epimerase from Brucella abortus

Released:
Source organism: Brucella abortus 2308
Entry authors: Horanyi PS, Abendroth J, Lorimer D, Edwards T, Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed:
UDP-alpha-D-glucose = UDP-alpha-D-galactose
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucose 4-epimerase Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 36.26 KDa
Source organism: Brucella abortus 2308
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q2YKG6 (Residues: 1-328; Coverage: 100%)
Gene names: BAB2_0694, galE-2
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: I222
Unit cell:
a: 76.6Å b: 86.11Å c: 126.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.178 0.214
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'