› 4twf

X-ray diffraction
3.9Å resolution

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with bromomemantine

Released:
DOI: 10.2210/pdb4twf/pdb
PDB 4twf coloured by chain and viewed from the front.
PDB 4twf coloured by chain and viewed from the side.
PDB 4twf coloured by chain and viewed from the top.
Source organism: Dickeya chrysanthemi
Primary publication:
The Prokaryote Ligand-Gated Ion Channel ELIC Captured in a Pore Blocker-Bound Conformation by the Alzheimer's Disease Drug Memantine.
Ulens C, Spurny R, Thompson AJ, Alqazzaz M, Debaveye S, Han L, Price K, Villalgordo JM, Tresadern G, Lynch JW, Lummis SC
Structure (2014)
PMID: 25199693

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cys-loop ligand-gated ion channel Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 307 amino acids
Theoretical weight: 35.28 KDa
Source organism: Dickeya chrysanthemi
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C7B7 (Residues: 11-163, 164-316; Coverage: 95%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BR7 12 x BR7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 106.505Å b: 266.092Å c: 112.175Å
α: 90° β: 107.7° γ: 90°
R-values:
R R work R free
0.204 0.202 0.25
Expression system: Escherichia coli

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Citations

1 review citation

Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins.
Hanashima et al. (2021)
6 other articles

2 mentions without citation

Structure and Pharmacologic Modulation of Inhibitory Glycine Receptors.
Burgos et al. (2016)
1 more