4tur

X-ray diffraction
2.17Å resolution

Human DNA polymerase beta inserting dCMPNPP opposite the 5'G of cisplatin crosslinked Gs (Pt-GG2)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase beta Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 37.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06746 (Residues: 7-335; Coverage: 98%)
Gene name: POLB
Sequence domains:
Structure domains:
DNA (5'-D(*CP*CP*CP*AP*CP*GP*GP*CP*CP*CP*AP*TP*CP*AP*CP*C)-3') Chain: T
Molecule details ›
Chain: T
Length: 16 nucleotides
Theoretical weight: 4.75 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*GP*GP*TP*GP*AP*TP*GP*GP*GP*C)-3') Chain: P
Molecule details ›
Chain: P
Length: 10 nucleotides
Theoretical weight: 3.14 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*GP*TP*GP*GP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 5 nucleotides
Theoretical weight: 1.58 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 54.785Å b: 79.335Å c: 54.786Å
α: 90° β: 107.81° γ: 90°
R-values:
R R work R free
0.205 0.202 0.256
Expression systems:
  • Escherichia coli
  • Not provided