› 4tsr

X-ray diffraction
2.07Å resolution

The Complex Structure of Mutant Phytase with IHS

Released:
DOI: 10.2210/pdb4tsr/pdb
PDB 4tsr coloured by chain and viewed from the front.
PDB 4tsr coloured by chain and viewed from the side.
PDB 4tsr coloured by chain and viewed from the top.
Source organism: Escherichia coli
Entry authors: Wu TH, Chen CC, Huang CH, Guo RT

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phytase AppA Chain: A
Molecule details ›
Chain: A
Length: 410 amino acids
Theoretical weight: 44.75 KDa
Source organism: Escherichia coli
Expression system: Komagataella phaffii CBS 7435
UniProt:
  • Canonical: P07102 (Residues: 23-432; Coverage: 100%)
Gene names: JW0963, appA, b0980
Sequence domains: Histidine phosphatase superfamily (branch 2)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

2 bound ligands:
Ligand NI 4 x NI
Ligand IHS 1 x IHS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P21
Unit cell:
a: 63.796Å b: 47.5Å c: 65.632Å
α: 90° β: 100.51° γ: 90°
R-values:
R R work R free
0.174 0.172 0.218
Expression system: Komagataella phaffii CBS 7435

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