X-ray diffraction
1.8Å resolution

Crystal structure of DNA polymerase sliding clamp from Pseudomonas aeruginosa


Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Beta sliding clamp Chains: A, B
Molecule details ›
Chains: A, B
Length: 383 amino acids
Theoretical weight: 42.55 KDa
Source organism: Pseudomonas aeruginosa HB15
Expression system: Escherichia coli
  • Canonical: Q9I7C4 (Residues: 1-367; Coverage: 100%)
Gene names: PA0002, dnaN
Sequence domains:
Structure domains: DNA Polymerase III, subunit A, domain 2

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 80.53Å b: 92.41Å c: 127.4Å
α: 90° β: 90° γ: 90°
R R work R free
0.209 0.207 0.232
Expression system: Escherichia coli