X-ray diffraction
2.29Å resolution

Crystal structure of DNA polymerase sliding clamp from Mycobaterium tuberculosis


Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Beta sliding clamp Chains: A, B
Molecule details ›
Chains: A, B
Length: 402 amino acids
Theoretical weight: 42.15 KDa
Source organism: Mycobacterium tuberculosis CDC1551
Expression system: Escherichia coli
  • Canonical: P9WNU0 (Residues: 1-402; Coverage: 100%)
Gene names: MT0002, dnaN
Sequence domains:
Structure domains: DNA Polymerase III, subunit A, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P1
Unit cell:
a: 40.49Å b: 83.65Å c: 84.03Å
α: 63.58° β: 86.84° γ: 85.2°
R R work R free
0.204 0.202 0.238
Expression system: Escherichia coli