› 4tqq

X-ray diffraction
2.5Å resolution

Photosynthetic Reaction Center from R. sphaeroides Analyzed at Room Temperature on an X-ray Transparent Microfluidic Chip

Released:
DOI: 10.2210/pdb4tqq/pdb
PDB entry 4tqq coloured by chain, front view.
PDB entry 4tqq coloured by chain, side view.
PDB entry 4tqq coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
X-ray Transparent Microfluidic Chip for Mesophase-Based Crystallization of Membrane Proteins and On-Chip Structure Determination.
Khvostichenko DS, Schieferstein JM, Pawate AS, Laible PD, Kenis PJ
Cryst Growth Des 14 4886-4890 (2014)
PMID: 25285049

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142934 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 240 amino acids
Theoretical weight: 26.02 KDa
Source organism: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 11-250; Coverage: 92%)
Gene name: puhA
Sequence domains:
Structure domains:
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 281 amino acids
Theoretical weight: 31.35 KDa
Source organism: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 2-282; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 302 amino acids
Theoretical weight: 33.87 KDa
Source organism: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 2-303; Coverage: 98%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like

Ligands and Environments

5 bound ligands:
Ligand BPH 2 x BPH
Ligand BCL 4 x BCL
Ligand UQ1 1 x UQ1
Ligand FE2 1 x FE2
Ligand U10 1 x U10
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P42212
Unit cell:
a: 102.484Å b: 102.484Å c: 239.939Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.213

Quick links

Citations

4 review citations

Recent advances in X-ray compatible microfluidics for applications in soft materials and life sciences.
Ghazal et al. (2016)
3 more

3 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
2 more