4tqf

X-ray diffraction
2.71Å resolution

Crystal Structure of the C-terminal domain of IFRS bound with 2-(5-bromothienyl)-L-Ala and ATP

Released:
Source organism: Methanosarcina mazei Go1
Entry authors: Nakamura A, O'Donoghue P, Soll D

Function and Biology Details

Reaction catalysed:
ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl-tRNA(Pyl)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyrrolysine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 33.4 KDa
Source organism: Methanosarcina mazei Go1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8PWY1 (Residues: 185-454; Coverage: 60%)
Gene names: MM_1445, pylS
Sequence domains: tRNA synthetases class II core domain (F)
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P64
Unit cell:
a: 105.11Å b: 105.11Å c: 70.29Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.168 0.211
Expression system: Escherichia coli BL21(DE3)