› 4tpg

X-ray diffraction
3.91Å resolution

Selectivity mechanism of a bacterial homologue of the human drug peptide transporters PepT1 and PepT2

Released:
DOI: 10.2210/pdb4tpg/pdb
PDB 4tpg coloured by chain and viewed from the front.
PDB 4tpg coloured by chain and viewed from the side.
PDB 4tpg coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Selectivity mechanism of a bacterial homolog of the human drug-peptide transporters PepT1 and PepT2.
Guettou F, Quistgaard EM, Raba M, Moberg P, Löw C, Nordlund P
Nat. Struct. Mol. Biol. 21 728-31 (2014)
PMID: 25064511

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proton:oligopeptide symporter POT family Chains: A, B
Molecule details ›
Chains: A, B
Length: 523 amino acids
Theoretical weight: 57.22 KDa
Source organism: Shewanella oneidensis MR-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8EHE6 (Residues: 1-516; Coverage: 100%)
Gene name: SO_1277
Sequence domains: POT family
Structure domains: MFS general substrate transporter like domains
Ala-L-3-Br-Tyr-Ala Chain: E
Molecule details ›
Chain: E
Length: 3 amino acids
Theoretical weight: 481 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand LMT 2 x LMT
1 modified residue:
Ligand DBY 1 x DBY

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 82.89Å b: 108.09Å c: 206.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.262 0.261 0.29
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Function, Regulation, and Pathophysiological Relevance of the POT Superfamily, Specifically PepT1 in Inflammatory Bowel Disease.
Viennois et al. (2018)
6 more

4 mentions without citation

Recent advances in understanding prodrug transport through the SLC15 family of proton-coupled transporters.
Minhas et al. (2020)
3 more