› 4to0

X-ray diffraction
2.3Å resolution

Structure basis of cellular dNTP regulation, SAMHD1-GTP-dATP-dCTP complex

Released:
DOI: 10.2210/pdb4to0/pdb
PDB 4to0 coloured by chain and viewed from the front.
PDB 4to0 coloured by chain and viewed from the side.
PDB 4to0 coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Structural basis of cellular dNTP regulation by SAMHD1.
Ji X, Tang C, Zhao Q, Wang W, Xiong Y
Proc. Natl. Acad. Sci. U.S.A. (2014)
PMID: 25267621

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 514 amino acids
Theoretical weight: 59.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y3Z3 (Residues: 113-626; Coverage: 82%)
Gene names: MOP5, SAMHD1
Sequence domains: HD domain
Structure domains: Hypothetical protein af1432

Ligands and Environments

4 bound ligands:
Ligand DCP 4 x DCP
Ligand GTP 4 x GTP
Ligand DTP 4 x DTP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 81.418Å b: 141.329Å c: 97.519Å
α: 90° β: 114.58° γ: 90°
R-values:
R R work R free
0.193 0.191 0.222
Expression system: Escherichia coli

Quick links

Citations

13 review citations

SAMHD1 in cancer: curse or cure?
Schott et al. (2022)
12 more

2 mentions without citation

HIV suppression by host restriction factors and viral immune evasion.
Jia et al. (2015)
1 more