› 4tnw

X-ray diffraction
3.2Å resolution

C. elegans glutamate-gated chloride channel (GluCl) in complex with Fab and POPC in a lipid-modulated conformation

Released:
DOI: 10.2210/pdb4tnw/pdb
PDB entry 4tnw coloured by chain, front view.
PDB entry 4tnw coloured by chain, side view.
PDB entry 4tnw coloured by chain, top view.
Source organisms:
Primary publication:
X-ray structures of GluCl in apo states reveal a gating mechanism of Cys-loop receptors.
Althoff T, Hibbs RE, Banerjee S, Gouaux E
Nature 512 333-7 (2014)
PMID: 25143115

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229719 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Glutamate-gated chloride channel alpha Chains: A, B, C, D, E, P, Q, R, S, T
Molecule details ›
Chains: A, B, C, D, E, P, Q, R, S, T
Length: 347 amino acids
Theoretical weight: 39.64 KDa
Source organism: Caenorhabditis elegans
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: G5EBR3 (Residues: 62-363, 422-455; Coverage: 76%)
Gene names: F11A5.10, glc-1
Sequence domains:
Structure domains:
Mouse monoclonal Fab fragment, heavy chain Chains: F, G, H, I, J, U, V, W, X, Y
Molecule details ›
Chains: F, G, H, I, J, U, V, W, X, Y
Length: 224 amino acids
Theoretical weight: 24.3 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Mouse monoclonal Fab fragment, light chain Chains: K, L, M, N, O, Z, f, g, h, i
Molecule details ›
Chains: K, L, M, N, O, Z, f, g, h, i
Length: 215 amino acids
Theoretical weight: 23.15 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

5 bound ligands:
Ligand NAG 9 x NAG
Ligand POV 7 x POV
Ligand CL 2 x CL
Ligand GOL 1 x GOL
Ligand LMT 7 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 453.75Å b: 192.87Å c: 196.14Å
α: 90° β: 92.27° γ: 90°
R-values:
R R work R free
0.228 0.227 0.251
Expression system: Spodoptera frugiperda

Quick links

Citations

31 review citations

Allosteric Modulation as a Unifying Mechanism for Receptor Function and Regulation.
Changeux et al. (2016)
30 more

16 mentions without citation

Druggable Lipid Binding Sites in Pentameric Ligand-Gated Ion Channels and Transient Receptor Potential Channels.
Cheng et al. (2021)
15 more