› 4tnq

X-ray diffraction
2.55Å resolution

Structural basis of cellular dNTP regulation, SAMHD1-GTP-dTTP-dTTP complex

Released:
DOI: 10.2210/pdb4tnq/pdb
PDB 4tnq coloured by chain and viewed from the front.
PDB 4tnq coloured by chain and viewed from the side.
PDB 4tnq coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Structural basis of cellular dNTP regulation by SAMHD1.
Ji X, Tang C, Zhao Q, Wang W, Xiong Y
Proc. Natl. Acad. Sci. U.S.A. (2014)
PMID: 25267621

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 514 amino acids
Theoretical weight: 59.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y3Z3 (Residues: 113-626; Coverage: 82%)
Gene names: MOP5, SAMHD1
Sequence domains: HD domain
Structure domains: Hypothetical protein af1432

Ligands and Environments

3 bound ligands:
Ligand TTP 8 x TTP
Ligand GTP 4 x GTP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 81.159Å b: 140.292Å c: 97.268Å
α: 90° β: 114.56° γ: 90°
R-values:
R R work R free
0.163 0.161 0.198
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Mechanistic Interplay between HIV-1 Reverse Transcriptase Enzyme Kinetics and Host SAMHD1 Protein: Viral Myeloid-Cell Tropism and Genomic Mutagenesis.
Bowen et al. (2022)
15 more

2 mentions without citation

Structural basis of cellular dNTP regulation by SAMHD1.
Ji et al. (2014)
1 more