4tn2

X-ray diffraction
2.7Å resolution

NS5b in complex with lactam-thiophene carboxylic acids

Released:

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 577 amino acids
Theoretical weight: 64.19 KDa
Source organism: Hepatitis B virus
UniProt:
  • Canonical: P26663 (Residues: 2422-2989; Coverage: 19%)
Sequence domains: Viral RNA dependent RNA polymerase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E DW
Spacegroup: P65
Unit cell:
a: 159.191Å b: 159.191Å c: 71.226Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.22 0.247