› 4tl9

X-ray diffraction
1.82Å resolution

Crystal structure of N-terminal C1 domain of KaiC

Released:
DOI: 10.2210/pdb4tl9/pdb
PDB entry 4tl9 coloured by chain, front view.
PDB entry 4tl9 coloured by chain, side view.
PDB entry 4tl9 coloured by chain, top view.
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Primary publication:
Circadian rhythms. Atomic-scale origins of slowness in the cyanobacterial circadian clock.
Abe J, Hiyama TB, Mukaiyama A, Son S, Mori T, Saito S, Osako M, Wolanin J, Yamashita E, Kondo T, Akiyama S
Science 349 312-6 (2015)
PMID: 26113637

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Circadian clock oscillator protein KaiC Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 253 amino acids
Theoretical weight: 28.39 KDa
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Expression system: Escherichia coli
UniProt:
  • Canonical: Q79PF4 (Residues: 1-253; Coverage: 49%)
Gene names: Synpcc7942_1216, kaiC, see0011
Sequence domains: KaiC
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 6 x MG
Ligand CL 6 x CL
Ligand AGS 6 x AGS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 80.134Å b: 133.82Å c: 151.167Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.217
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Circadian Oscillators: Around the Transcription-Translation Feedback Loop and on to Output.
Hurley et al. (2016)
14 more

1 mention without citation

Circadian oscillator proteins across the kingdoms of life: structural aspects.
Saini et al. (2019)