› 4tks

X-ray diffraction
3.2Å resolution

Native-SAD phasing for human EGFR kinase domain.

Released:
DOI: 10.2210/pdb4tks/pdb
PDB entry 4tks coloured by chain, front view.
PDB entry 4tks coloured by chain, side view.
PDB entry 4tks coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Multi-crystal native SAD analysis at 6 keV.
Liu Q, Guo Y, Chang Y, Cai Z, Assur Z, Mancia F, Greene MI, Hendrickson WA
Acta Crystallogr D Biol Crystallogr 70 2544-57 (2014)
PMID: 25286840

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132671 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Epidermal growth factor receptor Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 37.71 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00533 (Residues: 695-1022; Coverage: 28%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: I23
Unit cell:
a: 146.161Å b: 146.161Å c: 146.161Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.209
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Crystallographic phasing from weak anomalous signals.
Liu et al. (2015)
1 more

5 mentions without citation

Why Some Targets Benefit from beyond Rule of Five Drugs.
Egbert et al. (2019)
4 more