› 4sli

X-ray diffraction
1.8Å resolution

LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2-PROPENYL-NEU5AC, AN INACTIVE SUBSTRATE ANALOGUE

Released:
DOI: 10.2210/pdb4sli/pdb
PDB entry 4sli coloured by chain, front view.
PDB entry 4sli coloured by chain, side view.
PDB entry 4sli coloured by chain, top view.
Source organism: Macrobdella decora
Primary publication:
The 1.8 A structures of leech intramolecular trans-sialidase complexes: evidence of its enzymatic mechanism.
Luo Y, Li SC, Li YT, Luo M
J Mol Biol 285 323-32 (1999)
PMID: 9878409

Function and Biology Details

Reaction catalysed: 
Elimination of alpha-sialyl groups in N-acetylneuraminic acid glycosides, releasing 2,7-anhydro-alpha-N-acetylneuraminate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173277 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anhydrosialidase Chain: A
Molecule details ›
Chain: A
Length: 679 amino acids
Theoretical weight: 74.57 KDa
Source organism: Macrobdella decora
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q27701 (Residues: 81-759; Coverage: 92%)
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CNP 1 x CNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P1
Unit cell:
a: 46.264Å b: 69.437Å c: 72.319Å
α: 112.93° β: 95.6° γ: 106.86°
R-values:
R R work R free
0.189 0.189 0.229
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Exploration of the Sialic Acid World.
Schauer et al. (2018)
3 more