X-ray diffraction
1.64Å resolution

Crystal structure of Staphylococcal nuclease variant Delta+PHS I92K/V23A at cryogenic temperature

Source organism: Staphylococcus aureus
Entry authors: Caro JA, Sue G, Schlessman JL, Heroux A, Garcia-Moreno E B

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.13 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P00644 (Residues: 83-231; Coverage: 70%)
Gene name: nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 31.112Å b: 59.956Å c: 38.291Å
α: 90° β: 94.72° γ: 90°
R R work R free
0.177 0.173 0.216
Expression system: Escherichia coli BL21(DE3)