4s3g

X-ray diffraction
2.5Å resolution

Structure of the F249X mutant of Phosphatidylinositol-specific phospholipase C from Staphylococcus aureus

Released:

Function and Biology Details

Reaction catalysed:
1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.26 KDa
Source organism: Staphylococcus aureus subsp. aureus str. Newman
Expression system: Escherichia coli
UniProt:
  • Canonical: P45723 (Residues: 11-312; Coverage: 100%)
Gene names: NWMN_0041, plc
Sequence domains: Phosphatidylinositol-specific phospholipase C, X domain
Structure domains: Phosphatidylinositol (PI) phosphodiesterase

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P43212
Unit cell:
a: 60.05Å b: 60.05Å c: 191.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.212 0.291
Expression system: Escherichia coli