4s36

X-ray diffraction
1.46Å resolution

Crystal structure of the C-terminal domain of R2 pyocin membrane-piercing spike

Released:
Source organism: Pseudomonas aeruginosa
Entry authors: Browning CB, Leiman PG, Shneider MM

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phage_base_V domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 9.43 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9S581 (Residues: 96-185; Coverage: 49%)
Gene names: E4V10_20340, IPC1164_04680, IPC737_32475, IPC90_06695, PA52Ts2_5703, RW109_RW109_01282, VR2

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P6322
Unit cell:
a: 46.205Å b: 46.205Å c: 144.954Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.151 0.149 0.188
Expression system: Escherichia coli BL21(DE3)