› 4s33

X-ray diffraction
1.48Å resolution

ERK2 R65S mutant complexed with AMP-PNP

Released:
DOI: 10.2210/pdb4s33/pdb
PDB 4s33 coloured by chain and viewed from the front.
PDB 4s33 coloured by chain and viewed from the side.
PDB 4s33 coloured by chain and viewed from the top.
Source organism: Rattus norvegicus
Entry authors: Livnah O, Karamansha Y, Tzarum N

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 358 amino acids
Theoretical weight: 41.26 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63086 (Residues: 1-358; Coverage: 100%)
Gene names: Erk2, Mapk, Mapk1, Prkm1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ANP 1 x ANP
Ligand SO4 1 x SO4
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 48.813Å b: 70.438Å c: 60.082Å
α: 90° β: 109.28° γ: 90°
R-values:
R R work R free
0.199 0.196 0.239
Expression system: Escherichia coli

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