X-ray diffraction
2.93Å resolution

Crystal structure of Caulobacter crescentus ThiC with Fe4S4 cluster at remote site (holo form)


Function and Biology Details

Reaction catalysed:
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphomethyl)pyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Phosphomethylpyrimidine synthase Chain: A
Molecule details ›
Chain: A
Length: 614 amino acids
Theoretical weight: 68.25 KDa
Source organism: Caulobacter vibrioides CB15
Expression system: Escherichia coli
  • Canonical: Q9A6Q5 (Residues: 1-612; Coverage: 100%)
Gene names: CC_2029, thiC
Sequence domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 116.657Å b: 68.922Å c: 97.62Å
α: 90° β: 120.3° γ: 90°
R R work R free
0.197 0.194 0.246
Expression system: Escherichia coli