› 4s1h

X-ray diffraction
1.6Å resolution

Pyridoxal kinase of Entamoeba histolytica with ADP

Released:
DOI: 10.2210/pdb4s1h/pdb
PDB 4s1h coloured by chain and viewed from the front.
PDB 4s1h coloured by chain and viewed from the side.
PDB 4s1h coloured by chain and viewed from the top.
Source organism: Entamoeba histolytica
Primary publication:
Characterization and functional insights into the Entamoeba histolytica pyridoxal kinase, an enzyme essential for its survival.
Tarique KF, Devi S, Tomar P, Ali MF, Rehman SAA, Gourinath S
J Struct Biol 212 107645 (2020)
PMID: 33045383

Function and Biology Details

Reaction catalysed: 
ATP + pyridoxal = ADP + pyridoxal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
pyridoxal kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 287 amino acids
Theoretical weight: 32.1 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: C4LVZ4 (Residues: 1-279; Coverage: 100%)
Gene name: EHI_126090
Sequence domains: Phosphomethylpyrimidine kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P1
Unit cell:
a: 44.256Å b: 44.272Å c: 75.246Å
α: 89.94° β: 102.89° γ: 107.03°
R-values:
R R work R free
0.189 0.187 0.219
Expression system: Escherichia coli BL21

Quick links

Citations

2 citation in other articles

Virtual Screening of FDA-Approved Drugs against Triose Phosphate Isomerase from Entamoeba histolytica and Giardia lamblia Identifies Inhibitors of Their Trophozoite Growth Phase.
Juárez-Saldivar et al. (2021)
1 more