X-ray diffraction
1.5Å resolution

Crystal Structure of HLTF HIRAN Domain bound to DNA


Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Helicase-like transcription factor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 130 amino acids
Theoretical weight: 14.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q14527 (Residues: 55-180; Coverage: 13%)
Gene names: HIP116A, HLTF, RNF80, SMARCA3, SNF2L3, ZBU1
Sequence domains: HIRAN domain
5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3' Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 10 nucleotides
Theoretical weight: 3 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 61.026Å b: 74.211Å c: 66.18Å
α: 90° β: 113.68° γ: 90°
R R work R free
0.154 0.153 0.185
Expression systems:
  • Escherichia coli
  • Not provided