4s0k

X-ray diffraction
2.1Å resolution

Biphenylalanine modified threonyl-tRNA synthetase from Pyrococcus abyssi: 11BIF, 42F, 79V, and 123A mutant

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Threonine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 151 amino acids
Theoretical weight: 17.33 KDa
Source organism: Pyrococcus abyssi GE5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UZ14 (Residues: 1-143; Coverage: 23%)
Gene names: PAB1490, PYRAB13430, thrS
Sequence domains: Archaea-specific editing domain of threonyl-tRNA synthetase
Structure domains: D-tyrosyl-tRNA(Tyr) deacylase

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I222
Unit cell:
a: 52.43Å b: 79.15Å c: 90.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.212
Expression system: Escherichia coli BL21(DE3)