X-ray diffraction
1.98Å resolution

Structural Analysis of Substrate, Reaction Intermediate and Product Binding in Haemophilus influenzae Biotin Carboxylase


Function and Biology Details

Reactions catalysed:
ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA
ATP + [biotin carboxyl-carrier protein]-biotin-N(6)-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N(6)-L-lysine
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Biotin carboxylase Chain: A
Molecule details ›
Chain: A
Length: 468 amino acids
Theoretical weight: 51.35 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli
  • Canonical: P43873 (Residues: 1-448; Coverage: 100%)
Gene names: HI_0972, accC
Sequence domains:
Structure domains:

Ligands and Environments

Cofactor: Ligand Y7Y 1 x Y7Y
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P64
Unit cell:
a: 86.197Å b: 86.197Å c: 102.364Å
α: 90° β: 90° γ: 120°
R R work R free
0.178 0.176 0.217
Expression system: Escherichia coli