› 4rz2

X-ray diffraction
2.8Å resolution

Crystal structure of the MinD-like ATPase FlhG

Released:
DOI: 10.2210/pdb4rz2/pdb
PDB entry 4rz2 coloured by chain, front view.
PDB entry 4rz2 coloured by chain, side view.
PDB entry 4rz2 coloured by chain, top view.
Source organism: Geobacillus thermodenitrificans NG80-2
Primary publication:
MinD-like ATPase FlhG effects location and number of bacterial flagella during C-ring assembly.
Schuhmacher JS, Rossmann F, Dempwolff F, Knauer C, Altegoer F, Steinchen W, Dörrich AK, Klingl A, Stephan M, Linne U, Thormann KM, Bange G
Proc Natl Acad Sci U S A 112 3092-7 (2015)
PMID: 25733861

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107344 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flagellar synthesis regulator fleN Chains: A, B
Molecule details ›
Chains: A, B
Length: 295 amino acids
Theoretical weight: 32.66 KDa
Source organism: Geobacillus thermodenitrificans NG80-2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4IMB4 (Residues: 1-287; Coverage: 100%)
Gene name: GTNG_1094
Sequence domains: NUBPL iron-transfer P-loop NTPase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 63.87Å b: 89.36Å c: 111.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.177 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Type III secretion systems: the bacterial flagellum and the injectisome.
Diepold et al. (2015)
5 more

3 mentions without citation

Structural Conservation and Adaptation of the Bacterial Flagella Motor.
Carroll et al. (2020)
2 more